Identifying Common Genetic Variants by High-Resolution Melting
نویسندگان
چکیده
منابع مشابه
Identifying common genetic variants by high-resolution melting.
BACKGROUND Heteroduplex scanning techniques usually detect all heterozygotes, including common variants not of clinical interest. METHODS We conducted high-resolution melting analysis on the 24 exons of the ACVRL1 and ENG genes implicated in hereditary hemorrhagic telangiectasia (HHT). DNA in samples from 13 controls and 19 patients was PCR amplified in the presence of LCGreen I, and all 768 ...
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Genotyping by amplicon melting is a minimalistic approach to geno-typing, requiring only PCR, a fluorescent DNA dye, and single-color acquisition of a melting curve in a closed tube. Multiplex genotyping for the F5 1 [coagulation factor V (proaccelerin, labile factor)] Le-iden (1691GϾA), F2 [coagulation factor II (thrombin)] 20210GϾA, and MTHFR [methylenetetrahy-drofolate reductase (NAD(P)H)] 1...
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Currently, the widely used automated capillary electrophoresis-based short tandem repeat (STR) genotyping method for genetic screening in forensic practice is laborious, time-consuming, expensive, and technically challenging in some cases. Thus, new molecular-based strategies for conclusively identifying forensically relevant biological evidence are required. Here, we used high-resolution melti...
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Background: Colorectal cancer (CRC) is the third most common cancer worldwide. microsatellite instability (MSI) is a molecular marker of a deficient mismatch repair system and happens in almost 15% of CRCs. Because of a wide frequency of MSI+ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detecti...
متن کاملMultiplex amplicon genotyping by high-resolution melting.
High-resolution amplicon melting is a simple method for genotyping that uses only generic PCR primers and a saturating DNA dye. Multiplex amplicon genotyping has previously been reported in a single color, but two instruments were required: a carousel-based rapid cycler and a high-resolution melting instrument for capillaries. Manual transfer of capillaries between instruments and sequential me...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2007
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2007.085407